The automated determination of NAD-coupled enzymes. II. Serum lactate dehydrogenase.
نویسندگان
چکیده
A precise automated procedure developed for determination of serum lactate dehydrogenase, using the Robot Chemist, measures absorbance of a cuprous-neocuproine complex formed by coupled reduction of the cupric-neocuproine reagent with enzymatically generated reduced nicotinamide adenine dinucleotide. Activity values obtained by this method, by the identical automated colorimetric method on the AutoAnalyzer, and by an automated spectrophotometric (34O-m ) procedure show excellent agreement.
منابع مشابه
COFACTOR-SUBSTRATE ELUTION OF ENZYMES BOUND TO THE IMMOBILIZED NUCLEOTIDES ADENOSINE 5'-MONOPHOSPHATE AND NICOTINAMIDE-ADENINE DINUCLEOTIDE By K. MOSBACH, H. GUILFORD, R. OHLSSON and M. SCOTT
1. Two different gels have been prepared suitable for the separation of a number of enzymes, in particular NAD+-dependent dehydrogenases, by affinity chromatography. For both the matrix used was Sepharose 4B. For preparation (a), NAD+-Sepharose, 6-aminohexanoic acid has been coupled to the gel by the cyanogen bromide method and then NADI was attached by using dicyclohexylcarbodi-imide; for prep...
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عنوان ژورنال:
- Clinical chemistry
دوره 12 5 شماره
صفحات -
تاریخ انتشار 1966